Sanger sequencing is also called the chain termination method. This is particularly an important method that helps in the proper determination of the DNA nucleotide sequence. The method of Sanger sequencing was first developed in the year 1977 by the two-time Nobel Laureate Frederick Sanger and therefore, it is named so. For many of the years, the Sanger Sequencing performed by the capillary electrophoresis was regarded as the gold standard. This mainly starts by having a short primer binding next to the desired region. Now, the primer will be further extended by the polymerase specifically in the presence of 4 nucleotides.

In this step, also there will be the addition of complementary nucleotide from the template DNA strand. Again, for finding out the proper composition of the DNA sequence, it is important to bring this reaction to a defined stop. This again helps in the identification of the base at the end portion of a specific fragment of DNA. 16s Sequencing performs this step by removal of an oxygen atom specifically from the ribonucleotide. This particular nucleotide is known as the dideoxynucleotide.

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